Depth perception of stereoscopic transparent stimuli with frame manipulation.

Depth perception is crucial in human vision, allowing us to move and interact with our 3-D surroundings. We used a stereoscopic transparent stimulus comprising parallel overlapping transparent stereoscopic surfaces (POTS) to understand depth perception better. The study focused on exploring the effect of a surrounding frame on the perceived depth of a POTS configuration. The research was based on a proposed idea that explains an "off-frame" effect: a frame at a different depth from a 2-D photograph depicting a 3-D scene increases its apparent depth qualitatively. The idea assumes that processing the disparity between a frame and a photo reduces the reliability of the photograph's flatness cues and increases depth magnitude in depth cue integration. We examined whether the idea can be applied to a 3-D POTS with the flatness cue as the constant accommodation. Through three experiments, the study showed that frames impact the perceived depth magnitude of a POTS configuration. More specifically, the depth magnitude increases as the frame's disparity concerning the monitor plane increases and decreases as the frame's size increases. We discussed the results in the context of depth cue combination.

Effect of 3-D depth structure, element size, and area containing elements on total-element overestimation phenomenon.

The number of elements distributed in a three-dimensional stimulus is overestimated compared to a two-dimensional stimulus when both stimuli have the same number of elements. We examined the effect of the properties of a three-dimensional stimulus (the number of overlapping stereo surfaces, size of the elements, and size of the area containing elements, on the overestimation phenomenon in four experiments. The two stimuli were presented side-by-side with the same diameters. Observers judged which of the three-dimensional standard and two-dimensional comparison had more elements. The results showed that (a) the overestimation phenomenon occurred for the three-dimensional standard stimuli, (b) the size of the areas affected the amount of overestimation, while the number of overlapping stereo surfaces and size of elements did not, and (c) the amount of overestimation increased when the stimuli included more than 100 elements. Implications of these findings were discussed in the framework of back-surface bias, occlusion, and disparity-processing interference models.

Numerosity Comparison in Three Dimensions in the Case of Low Numerical Values.

This study investigated the perception of numbers in humans in 3D stimuli. Recent research has shown that number processing relies on "number sense" for small values, in line with Weber's law. While previous studies have reported 3D numerosity overestimation mainly in higher numerical values, our experiment examined whether this phenomenon occurs at lower numerical values. We also explored whether the Weber ratio follows Weber's law when comparing 2D and 3D stimuli in terms of the number of elements. Observers were presented with pairs of stimuli on a monitor and were asked to identify the stimulus with a larger number of elements. Using the constant method, we calculated the point of subjective equality (PSE), just noticeable difference (JND), and Weber ratios from the collected data. As a result, it was confirmed that the phenomenon of over-estimation of 3D numerical values occurs even when the numerical values are small. Additionally, we observed that the Weber fraction adhered to Weber's law within the measured range. These findings contribute to the existing body of research, supporting the existence of distinct mechanisms for perceiving numerosity and density.

Deep Learning of Cancer Stem Cell Morphology Using Conditional Generative Adversarial Networks.

Deep-learning workflows of microscopic image analysis are sufficient for handling the contextual variations because they employ biological samples and have numerous tasks. The use of well-defined annotated images is important for the workflow. Cancer stem cells (CSCs) are identified by specific cell markers. These CSCs were extensively characterized by the stem cell (SC)-like gene expression and proliferation mechanisms for the development of tumors. In contrast, the morphological characterization remains elusive. This study aims to investigate the segmentation of CSCs in phase contrast imaging using conditional generative adversarial networks (CGAN). Artificial intelligence (AI) was trained using fluorescence images of the Nanog-Green fluorescence protein, the expression of which was maintained in CSCs, and the phase contrast images. The AI model segmented the CSC region in the phase contrast image of the CSC cultures and tumor model. By selecting images for training, several values for measuring segmentation quality increased. Moreover, nucleus fluorescence overlaid-phase contrast was effective for increasing the values. We show the possibility of mapping CSC morphology to the condition of undifferentiation using deep-learning CGAN workflows.

Interaction of disparity size and depth structure on perceived numerosity in a three-dimensional space.

The number of elements in two stereo-surfaces parallelly overlapped in depth is overestimated compared to that in a single flat surface, even when both have the same number of elements. Using stereoscopic pairs of elements, we evaluated two hypotheses on the overestimation: one that a higher-order process, forming a background surface, increases the number of perceived elements, and the other that the number of elements potentially occluded by the elements on a front surface is taken accounted for. The data from four experiments showed that (a) when binocular disparity between (or among) stereoscopic elements was small, the overestimation occurred for the stimuli we used-a two-surface-overlapping stimulus, where the likelihood for the process to operate was manipulated by changing the averaged luminance of each surface, a volumetric stimulus, where the likelihood for the background surface to be formed would decrease, and a two-non-overlapping-surface stimulus, where the surfaces in depth were not overlapped-, and (b) when binocular disparity was large, the overestimation occurred for the two-surfaces-overlapping stimulus, when the averaged luminance of the two surfaces were the same, and for the volumetric stimulus, but diminished for the surface-overlapping stimulus, when the averaged luminance differed between the surfaces and for the surfaces-non-overlapping stimulus. These results cannot be explained either hypothesis only. We explain the results by postulating that the sensory system processing disparities of elements interferes with that estimating the number of elements, resulting in an overestimation of the elements in a stereo-stimulus, and the disparity range within which the interference occurs may depend on the stimulus depth structure.

Difference between tablet methods and paper questionnaire methods of conducting a survey with community-dwelling elderly.

[Purpose] This study aimed to develop a tablet app that emulates paper questionnaires used in clinical care, and to verify the difference between the utility of tablet survey methods and paper questionnaire methods with elderly people. [Subjects and Methods] A tablet app was developed in the Java language. A questionnaire was provided to 30 community-dwelling elderly people. The subjects were randomly allocated to the group responding on the tablet (tablet group) or that responding to a paper-based questionnaire (questionnaire group). Assessed items included response time to questions, whether or not they had queries regarding the survey, and data input time. For the tablet group, a questionnaire was conducted regarding the operability of the tablet. [Results] There was no difference in response time between the two groups. Significantly more people in the tablet group had queries regarding the survey. Data input time was 426 seconds for the tablet group and 1268 seconds for the questionnaire group. In the survey regarding tablet operability, there were no negative opinions about the visibility of the screen. [Conclusion] Tablets can be used with elderly people to shorten the data input time. The present findings suggested that tablet surveys could be effective for a large-scale investigation.

Overestimation of the number of elements in a three-dimensional stimulus.

Observers' numerosity judgments in binocular stereopsis were examined in four experiments, using random-dot stereograms (RDSs) that depicted a two-dimensional (2-D) stimulus side-by-side with a three-dimensional (3-D) stimulus. When the RDSs were correctly fused, a single surface and two (or three) transparent surfaces were observed for the 2-D and 3-D stimuli, respectively. Observers completed a numerosity discrimination task, where they judged which of the two stimuli had a greater number of dot elements. Results showed that (a) the 3-D stimulus was judged to contain more elements than the 2-D stimulus, even when both had the same number of elements, (b) the amount of overestimation increased as a function of the number of elements and the binocular disparity between the front and back surfaces of the 3-D stimulus, (c) the ratio of the physical number of elements in the front surface to that in the back surface of the 3-D stimulus had no effect on the magnitude of overestimation, and (d) when the number of elements for the two surfaces were judged separately, the ratio had more effect on the judged number of elements in the back surface than in the front surface. These results indicate that the extent of overestimation in the numerosity judgment of a set of elements in a stimulus depends on the number of depth layers in which the elements are embedded.

Magnitude of perceived depth of multiple stereo transparent surfaces.

According to the geometric relational expression of binocular stereopsis, for a given viewing distance the magnitude of the perceived depth of objects would be the same, as long as the disparity magnitudes were the same. However, we found that this is not necessarily the case for random-dot stereograms that depict parallel, overlapping, transparent stereoscopic surfaces (POTS). The data from five experiments indicated that (1) the magnitude of perceived depth between the two outer surfaces of a three- or a four-POTS configuration can be smaller than that for an identical pair of stereo surfaces of a two-POTS configuration for the range of disparities that we used (5.2-19.4 arcmin); (2) this phenomenon can be observed irrespective of the total dot density of a POTS configuration, at least for the range that we used (1.1-3.3 dots/deg(2)); and (3) the magnitude of perceived depth between the two outer surfaces of a POTS configuration can be reduced as the total number of stereo surfaces is increased, up to four surfaces. We explained these results in terms of a higher-order process or processes, with an output representing perceived depth magnitude, which is weakened when the number of its surfaces is increased.